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13gs
From Proteopedia
| 13gs, resolution 1.90Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||||
| Gene: | GSTP1 (Homo sapiens) | ||||||||
| Activity: | Glutathione transferase, with EC number 2.5.1.18 | ||||||||
| Domains: | GST_C_Pi, GST_N_Pi, Gst, IGc, V-set, IGv | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
GLUTATHIONE S-TRANSFERASE COMPLEXED WITH SULFASALAZINE
Glutathione S -transferases (GSTs) play a pivotal role in the detoxification of foreign chemicals and toxic metabolites. They were originally termed ligandins because of their ability to bind large molecules (molecular masses >400 Da), possibly for storage and transport roles. The location of the ligandin site in mammalian GSTs is still uncertain despite numerous studies in recent years. Here we show by X-ray crystallography that the ligandin binding site in human pi class GST P1-1 occupies part of one of the substrate binding sites. This work has been extended to the determination of a number of enzyme complex crystal structures which show that very large ligands are readily accommodated into this substrate binding site and in all, but one case, causes no significant movement of protein side-chains. Some of these molecules make use of a hitherto undescribed binding site located in a surface pocket of the enzyme. This site is conserved in most, but not all, classes of GSTs suggesting it may play an important functional role.
The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site)., Oakley AJ, Lo Bello M, Nuccetelli M, Mazzetti AP, Parker MW, J Mol Biol. 1999 Aug 27;291(4):913-26. PMID:10452896
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
13GS is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Oakley AJ, Lo Bello M, Nuccetelli M, Mazzetti AP, Parker MW. The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site). J Mol Biol. 1999 Aug 27;291(4):913-26. PMID:10452896
Page seeded by OCA on Mon Feb 16 12:04:09 2009
