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|1cx0, resolution 2.30Å ()|
HEPATITIS DELTA VIRUS RIBOZYME
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Ferre-D'Amare AR, Zhou K, Doudna JA. Crystal structure of a hepatitis delta virus ribozyme. Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582 doi:http://dx.doi.org/10.1038/26912
- Ferre-D'Amare AR, Doudna JA. Crystallization and structure determination of a hepatitis delta virus ribozyme: use of the RNA-binding protein U1A as a crystallization module. J Mol Biol. 2000 Jan 21;295(3):541-56. PMID:10623545 doi:10.1006/jmbi.1999.3398
- Osipiuk J, Gornicki P, Maj L, Dementieva I, Laskowski R, Joachimiak A. Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold. Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1747-51. Epub 2001, Oct 25. PMID:11679764
- Hicks JM, Hsu VL. The extended left-handed helix: a simple nucleic acid-binding motif. Proteins. 2004 May 1;55(2):330-8. PMID:15048824 doi:10.1002/prot.10630
- Chen JH, Gong B, Bevilacqua PC, Carey PR, Golden BL. A catalytic metal ion interacts with the cleavage Site G.U wobble in the HDV ribozyme. Biochemistry. 2009 Feb 24;48(7):1498-507. PMID:19178151 doi:10.1021/bi8020108