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1ema
From Proteopedia
| 1ema, resolution 1.90Å () | |
|---|---|
| Non-Standard Residues: | , |
| Domains: | GFP |
| Resources: | FirstGlance, OCA, PDBsum, RCSB |
| Coordinates: | save as pdb, mmCIF, xml |
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima.
Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1EMA is a Single protein structure of sequence from Aequorea victoria. Additional information on 1EMA is available in a page on Green Fluorescent Protein (GFP) at the RCSB PDB Molecule of the Month. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075
Page seeded by OCA on Wed Jul 23 11:01:52 2008
