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1g7s
From Proteopedia
| 1g7s, resolution 2.00Å () | |||||||||
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| Ligands: | |||||||||
| Related: | 1g7r, 1g7t | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDP
X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:11114334
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1g7s is a 1 chain structure with sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
- Roll-Mecak A, Cao C, Dever TE, Burley SK. X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding. Cell. 2000 Nov 22;103(5):781-92. PMID:11114334
