1gpc

Structural highlights

Function

SSB_BPT4 Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Promotes also the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125).[HAMAP-Rule:MF_04152]^{[1] [2] [3] [4] [5]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Single-stranded DNA-binding protein 3D structures

References

1. ↑ Bleuit JS, Xu H, Ma Y, Wang T, Liu J, Morrical SW. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. PMID:11459967 doi:10.1073/pnas.131007498
2. ↑ Ma Y, Wang T, Villemain JL, Giedroc DP, Morrical SW. Dual functions of single-stranded DNA-binding protein in helicase loading at the bacteriophage T4 DNA replication fork. J Biol Chem. 2004 Apr 30;279(18):19035-45. PMID:14871889 doi:10.1074/jbc.M311738200
3. ↑ Borjac-Natour JM, Petrov VM, Karam JD. Divergence of the mRNA targets for the Ssb proteins of bacteriophages T4 and RB69. Virol J. 2004 Sep 17;1:4. PMID:15507125 doi:10.1186/1743-422X-1-4
4. ↑ Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380
5. ↑ Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380