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1m5o, resolution 2.20Å ()
Non-Standard Residues:
Gene: SNRPA (Homo sapiens)
Related: 1m5k, 1m5p, 1m5v
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml


Transition State Stabilization by a Catalytic RNA

Publication Abstract from PubMed

The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes.

Transition state stabilization by a catalytic RNA., Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR, Science. 2002 Nov 15;298(5597):1421-4. Epub 2002 Oct 10. PMID:12376595

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1m5o is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also


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