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1m90
From Proteopedia
Contents |
Co-crystal structure of CCA-Phe-caproic acid-biotin and sparsomycin bound to the 50S ribosomal subunit
The large ribosomal subunit catalyzes peptide bond formation and will do so by using small aminoacyl- and peptidyl-RNA fragments of tRNA. We have refined at 3-A resolution the structures of both A and P site substrate and product analogues, as well as an intermediate analogue, bound to the Haloarcula marismortui 50S ribosomal subunit. A P site substrate, CCA-Phe-caproic acid-biotin, binds equally to both sites, but in the presence of sparsomycin binds only to the P site. The CCA portions of these analogues are bound identically by either the A or P loop of the 23S rRNA. Combining the separate P and A site substrate complexes into one model reveals interactions that may occur when both are present simultaneously. The alpha-NH(2) group of an aminoacylated fragment in the A site forms one hydrogen bond with the N3 of A2486 (2451) and may form a second hydrogen bond either with the 2' OH of the A-76 ribose in the P site or with the 2' OH of A2486 (2451). These interactions position the alpha amino group adjacent to the carbonyl carbon of esterified P site substrate in an orientation suitable for a nucleophilic attack.
Structural insights into peptide bond formation., Hansen JL, Schmeing TM, Moore PB, Steitz TA, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11670-5. Epub 2002 Aug 16. PMID:12185246
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1m90 is a 31 chain structure with sequence from Haloarcula marismortui. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L10
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L34
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L7
Reference
- Hansen JL, Schmeing TM, Moore PB, Steitz TA. Structural insights into peptide bond formation. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11670-5. Epub 2002 Aug 16. PMID:12185246 doi:10.1073/pnas.172404099
- Amann BT, Worthington MT, Berg JM. A Cys3His zinc-binding domain from Nup475/tristetraprolin: a novel fold with a disklike structure. Biochemistry. 2003 Jan 14;42(1):217-21. PMID:12515557 doi:10.1021/bi026988m
