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1n32
From Proteopedia
Contents |
Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin
A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.
Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1n32 is a 23 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein S10
- Ribosomal protein S11
- Ribosomal protein S12
- Ribosomal protein S13
- Ribosomal protein S14
- Ribosomal protein S15
- Ribosomal protein S16
- Ribosomal protein S17
- Ribosomal protein S18
- Ribosomal protein S19
- Ribosomal protein S2
- Ribosomal protein S20
- Ribosomal protein S3
- Ribosomal protein S4
- Ribosomal protein S5
- Ribosomal protein S6
- Ribosomal protein S7
- Ribosomal protein S8
- Ribosomal protein S9
- Ribosomal protein THX
- Ribosome
Reference
- Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V. Selection of tRNA by the ribosome requires a transition from an open to a closed form. Cell. 2002 Nov 27;111(5):721-32. PMID:12464183
Categories: Thermus thermophilus | IV, F V.Murphy. | Ogle, J M. | Ramakrishnan, V. | Tarry, M J. | 30s ribosomal subunit | A site | Antibiotic | Anticodon | Codon | Decoding | G:u | Gu | Messenger rna | Mismatch | Mrna | Near-cognate | Paromomycin | Ribosome | Stem-loop | Transfer rna | Trna | Wobble
