Structural highlights
1odz is a 2 chain structure with sequence from Cellvibrio japonicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.4Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
MANA_CELJU Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.,Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:12841226[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Braithwaite KL, Black GW, Hazlewood GP, Ali BR, Gilbert HJ. A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa. Biochem J. 1995 Feb 1;305 ( Pt 3):1005-10. PMID:7848261
- ↑ Halstead JR, Fransen MP, Eberhart RY, Park AJ, Gilbert HJ, Hazlewood GP. alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis. FEMS Microbiol Lett. 2000 Nov 15;192(2):197-203. PMID:11064195
- ↑ Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG. Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:12841226
