1q15

Structural highlights

Function

CARA_PECCC Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.^{[1] [2] [3]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Gerratana B, Stapon A, Townsend CA. Inhibition and alternate substrate studies on the mechanism of carbapenam synthetase from Erwinia carotovora. Biochemistry. 2003 Jul 1;42(25):7836-47. PMID:12820893 doi:http://dx.doi.org/10.1021/bi034361d
2. ↑ Arnett SO, Gerratana B, Townsend CA. Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase. Biochemistry. 2007 Aug 14;46(32):9337-45. Epub 2007 Jul 21. PMID:17658887 doi:http://dx.doi.org/10.1021/bi0618464
3. ↑ Raber ML, Arnett SO, Townsend CA. A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and beta-lactam synthetase. Biochemistry. 2009 Jun 9;48(22):4959-71. doi: 10.1021/bi900432n. PMID:19371088 doi:http://dx.doi.org/10.1021/bi900432n