1rcw

Structural highlights

Function

CADD_CHLTR Involved in de novo para-aminobenzoate (PABA) biosynthesis (PubMed:23972426, PubMed:32967910, PubMed:36122239). Acts as a self-sacrificing or 'suicide' enzyme that utilizes its own active site tyrosine residue(s) as the substrate for PABA synthesis (PubMed:32967910, PubMed:36122239). The side chain of the tyrosine residue is released from the protein backbone via cleavage of the C(alpha)-C(beta) bond, leaving a glycine in place of the original tyrosine residue (PubMed:32967910, PubMed:36122239). Reaction requires O(2) and a reduced dimetal cofactor (PubMed:32967910, PubMed:36122239).^{[1] [2] [3]} Was also identified as a specific toxin that associates with death domains of tumor necrosis factor family (TNF) receptors and induces apoptosis in mammalian cell lines through a Caspase-dependent mechanism.^{[4] [5]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Satoh Y, Kuratsu M, Kobayashi D, Dairi T. New gene responsible for para-aminobenzoate biosynthesis. J Biosci Bioeng. 2014 Feb;117(2):178-183. PMID:23972426 doi:10.1016/j.jbiosc.2013.07.013
2. ↑ Macias-Orihuela Y, Cast T, Crawford I, Brandecker KJ, Thiaville JJ, Murzin AG, de Crécy-Lagard V, White RH, Allen KD. An Unusual Route for p-Aminobenzoate Biosynthesis in Chlamydia trachomatis Involves a Probable Self-Sacrificing Diiron Oxygenase. J Bacteriol. 2020 Sep 23;202(20):e00319-20. PMID:32967910 doi:10.1128/JB.00319-20
3. ↑ Manley OM, Phan HN, Stewart AK, Mosley DA, Xue S, Cha L, Bai H, Lightfoot VC, Rucker PA, Collins L, Williams TI, Chang WC, Guo Y, Makris TM. Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para-aminobenzoate in Chlamydia trachomatis. Proc Natl Acad Sci U S A. 2022 Sep 27;119(39):e2210908119. PMID:36122239 doi:10.1073/pnas.2210908119
4. ↑ Stenner-Liewen F, Liewen H, Zapata JM, Pawlowski K, Godzik A, Reed JC. CADD, a Chlamydia protein that interacts with death receptors. J Biol Chem. 2002 Mar 22;277(12):9633-6. PMID:11805081 doi:10.1074/jbc.C100693200
5. ↑ Schwarzenbacher R, Stenner-Liewen F, Liewen H, Robinson H, Yuan H, Bossy-Wetzel E, Reed JC, Liddington RC. Structure of the Chlamydia protein CADD reveals a redox enzyme that modulates host cell apoptosis. J Biol Chem. 2004 Jul 9;279(28):29320-4. Epub 2004 Apr 15. PMID:15087448 doi:10.1074/jbc.M401268200