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|1gom, resolution 1.92Å ()|
|Related:||1fxm, 1gok, 1tax, 1tix|
THERMOSTABLE XYLANASE I FROM THERMOASCUS AURANTIACUS -CRYSTAL FORM I
The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX) has been investigated both biochemically and structurally. High resolution crystallographic analyses at 291 K and 100 K of TAX complexes with xylobiose show that the ligand is in its alpha anomeric conformation and provide a rationale for specificity on p-nitrophenyl glycosides at the -1 and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in family 10 are identified, which differ by the presence or absence of a short helical stretch in the eighth betaalpha-loop of the TIM barrel, the loop bearing Trp 275. This structural difference is discussed in the context of Trp 275 mobility and xylanase function.
Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A., Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S, FEBS Lett. 2001 Dec 7;509(2):303-8. PMID:11741607
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S. Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A. FEBS Lett. 2001 Dec 7;509(2):303-8. PMID:11741607
- Teixeira S, Lo Leggio L, Pickersgill R, Cardin C. Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I. Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):385-92. PMID:11223515
- Lo Leggio L, Kalogiannis S, Bhat MK, Pickersgill RW. High resolution structure and sequence of T. aurantiacus xylanase I: implications for the evolution of thermostability in family 10 xylanases and enzymes with (beta)alpha-barrel architecture. Proteins. 1999 Aug 15;36(3):295-306. PMID:10409823
- Pickersgill R, Harris G, Lo Leggio L, Mayans O, Jenkins J. Superfamilies: the 4/7 superfamily of beta alpha-barrel glycosidases and the right-handed parallel beta-helix superfamily. Biochem Soc Trans. 1998 May;26(2):190-8. PMID:9649746