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1w0n
From Proteopedia
| 1w0n, resolution 0.80Å () | |||||||||
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| Ligands: | , , | ||||||||
| Activity: | Endo-1,4-beta-xylanase, with EC number 3.2.1.8 | ||||||||
| Related: | 1ux7 | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36
The enzymatic degradation of polysaccharides harnesses multimodular enzymes whose carbohydrate binding modules (CBM) target the catalytic domain onto the recalcitrant substrate. Here we report the ab initio structure determination and subsequent refinement, at 0.8 A resolution, of the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity electrophoresis, isothermal titration calorimetry, and UV difference spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan binding domain. The 3D structure of CBM36 in complex with xylotriose and Ca(2+), at 1.5 A resolution, displays significant conformational changes compared to the native structure and reveals the molecular basis for its unique Ca(2+)-dependent binding of xylooligosaccharides through coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging spectrum of carbohydrate binding modules that increasingly find applications in industry and display great potential for mapping the "glyco-architecture" of plant cells.
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:15242594
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1w0n is a 1 chain structure with sequence from Paenibacillus polymyxa. Full crystallographic information is available from OCA.
See Also
Reference
- Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ. Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules. Structure. 2004 Jul;12(7):1177-87. PMID:15242594 doi:10.1016/j.str.2004.04.022
- Chen X, Weber I, Harrison RW. Hydration water and bulk water in proteins have distinct properties in radial distributions calculated from 105 atomic resolution crystal structures. J Phys Chem B. 2008 Sep 25;112(38):12073-80. Epub 2008 Aug 28. PMID:18754631 doi:10.1021/jp802795a
