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1wsu
From Proteopedia
Contents |
C-terminal domain of elongation factor selB complexed with SECIS RNA
SelB Recognition
| 1wsu, resolution 2.30Å () | |||||||||
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| Gene: | selB (Moorella thermoacetica) | ||||||||
| Domains: | PRK10512, SelB-wing_3 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Elongation factor SelB uses several methods to recognize the selenocysteine insertion sequence found in messenger RNA. The insertion sequence forms a hairpin loop with an unstacked guanine base in the loop. This guanine forms The planar base is also sandwiched between in the protein, which form a tight hydrophobic pocket. This is best seen using a The overall shape of the hairpin is recognized by contacts with several In particular, notice the intimate contact formed by both the charged nitrogen and hydrophobic carbon chain of
This page complements the article on Selenocysteine Synthase in the Molecule of the Month Series. See also Teaching Scenes, Tutorials, and Educators' Pages.
In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the molecular basis of which has been unknown. We have determined the crystal structure of the mRNA-binding domain of SelB in complex with SECIS RNA at a resolution of 2.3 A. This is the first example of a complex between an RNA and a winged-helix (WH) domain, a motif found in many DNA-binding proteins and recently discovered in RNA-binding proteins. Notably, RNA binding does not induce a major conformational change in the WH motif. The structure reveals a new mode of RNA recognition with a geometry that allows the complex to wrap around the small ribosomal subunit.
Structural basis for mRNA recognition by elongation factor SelB., Yoshizawa S, Rasubala L, Ose T, Kohda D, Fourmy D, Maenaka K, Nat Struct Mol Biol. 2005 Feb;12(2):198-203. Epub 2005 Jan 23. PMID:15665870
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1WSU is a Single protein structure of sequence from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
Structural basis for mRNA recognition by elongation factor SelB., Yoshizawa S, Rasubala L, Ose T, Kohda D, Fourmy D, Maenaka K, Nat Struct Mol Biol. 2005 Feb;12(2):198-203. Epub 2005 Jan 23. PMID:15665870
Page seeded by OCA on Tue Jul 22 21:24:18 2008
