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1zzn, resolution 3.37Å ()
Ligands: ,
Non-Standard Residues: , ,
Gene: SNRPA (Homo sapiens)
Related: 1u6b
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.

Publication Abstract from PubMed

We report the 3.4 angstrom crystal structure of a catalytically active group I intron splicing intermediate containing the complete intron, both exons, the scissile phosphate, and all of the functional groups implicated in catalytic metal ion coordination, including the 2'-OH of the terminal guanosine. This structure suggests that, like protein phosphoryltransferases, an RNA phosphoryltransferase can use a two-metal-ion mechanism. Two Mg2+ ions are positioned 3.9 angstroms apart and are directly coordinated by all six of the biochemically predicted ligands. The evolutionary convergence of RNA and protein active sites on the same inorganic architecture highlights the intrinsic chemical capacity of the two-metal-ion catalytic mechanism for phosphoryl transfer.

Structural evidence for a two-metal-ion mechanism of group I intron splicing., Stahley MR, Strobel SA, Science. 2005 Sep 2;309(5740):1587-90. PMID:16141079

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1zzn is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also


  • Stahley MR, Strobel SA. Structural evidence for a two-metal-ion mechanism of group I intron splicing. Science. 2005 Sep 2;309(5740):1587-90. PMID:16141079 doi:309/5740/1587
  • Adams PL, Stahley MR, Kosek AB, Wang J, Strobel SA. Crystal structure of a self-splicing group I intron with both exons. Nature. 2004 Jul 1;430(6995):45-50. Epub 2004 Jun 2. PMID:15175762 doi:10.1038/nature02642
  • Adams PL, Stahley MR, Gill ML, Kosek AB, Wang J, Strobel SA. Crystal structure of a group I intron splicing intermediate. RNA. 2004 Dec;10(12):1867-87. PMID:15547134 doi:10/12/1867
  • Cech TR, Zaug AJ, Grabowski PJ. In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence. Cell. 1981 Dec;27(3 Pt 2):487-96. PMID:6101203
  • Stahley MR, Adams PL, Wang J, Strobel SA. Structural metals in the group I intron: a ribozyme with a multiple metal ion core. J Mol Biol. 2007 Sep 7;372(1):89-102. Epub 2007 Jun 15. PMID:17612557 doi:10.1016/j.jmb.2007.06.026
  • Forconi M, Lee J, Lee JK, Piccirilli JA, Herschlag D. Functional identification of ligands for a catalytic metal ion in group I introns. Biochemistry. 2008 Jul 1;47(26):6883-94. Epub 2008 Jun 3. PMID:18517225 doi:10.1021/bi800519a

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