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2c38
From Proteopedia
| 2c38, resolution 3.10Å () | |||||||||
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| Ligands: | , | ||||||||
| Related: | 2br2, 2c37, 2c39 | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH A5 RNA
The exosome is a macromolecular complex that plays fundamental roles in the biogenesis and turnover of a large number of RNA species. Here we report the crystal structures of the Rrp41-Rrp42 core complex of the S. solfataricus exosome bound to short single-stranded RNAs and to ADP. The RNA binding cleft recognizes four nucleotides in a sequence-unspecific manner, mainly by electrostatic interactions with the phosphate groups. Interactions at the 2' hydroxyls of the sugars provide specificity for RNA over DNA. The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome.
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2c38 is a 24 chain structure with sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
- Lorentzen E, Conti E. Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core. Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928 doi:10.1016/j.molcel.2005.10.020
Categories: Sulfolobus solfataricus | Conti, E. | Lorentzen, E. | Archaeal | Exonuclease | Exoribonuclease | Exosome | Hydrolase | Nuclease | Phosphorolytic | Rna degradation | Rnase ph | Rrp41 | Rrp42
