2c5r
From Proteopedia
The structure of phage phi29 replication organizer protein p16.7 in complex with double stranded DNA
Structural highlights
FunctionGP167_BPPH2 Binds to the long stretches of ssDNA of the viral DNA replication intermediates created during the protein-primed mechanism of replication of the viral genome and attaches the viral DNA to the membrane of the infected cells (PubMed:11741949) (PubMed:11169113). Required for the redistribution of replicating viral DNA from the initial replication site to membrane-associated sites surrounding the nucleoid (PubMed:10921898). Required for the second pull step of DNA ejection (PubMed:17526715).[1] [2] [3] [4] Publication Abstract from PubMedProkaryotic DNA replication is compartmentalized at the cellular membrane. Functional and biochemical studies showed that the Bacillus subtilis phage 29-encoded membrane protein p16.7 is directly involved in the organization of membrane-associated viral DNA replication. The structure of the functional domain of p16.7 in complex with DNA, presented here, reveals the multimerization mode of the protein and provides insights in the organization of the phage genome at the membrane of the infected cell. Structural basis for membrane anchorage of viral phi29 DNA during replication.,Albert A, Munoz-Espin D, Jimenez M, Asensio JL, Hermoso JA, Salas M, Meijer WJ J Biol Chem. 2005 Dec 30;280(52):42486-8. Epub 2005 Nov 7. PMID:16275651[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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