First time at Proteopedia? Click on the green links, they change the 3D image. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more. Enter the Page of the Year Competition to win a 32GB iPod Touch and other prizes!
2fym
From Proteopedia
| 2fym, resolution 1.60Å () | |||||
|---|---|---|---|---|---|
| Ligands: | |||||
| Gene: | eno (Escherichia coli) | ||||
| Activity: | Phosphopyruvate hydratase, with EC number 4.2.1.11 | ||||
| Domains: | eno, enolase | ||||
| Related: | 1e9i | ||||
| |||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
Crystal structure of E. coli enolase complexed with the minimal binding segment of RNase E.
In Escherichia coli, the glycolytic enzyme enolase is a component of the RNA degradosome, which is an RNase E mediated assembly involved in RNA processing and transcript turnover. The recruitment of enolase by the RNA degradosome has been implicated in the turnover of certain transcripts, and it is mediated by a small segment of roughly a dozen residues that lie within a natively unstructured sub-domain of RNase E. Here, we present the crystal structure of enolase in complex with its recognition site from RNase E at 1.6A resolution. A single molecule of the RNase E peptide binds asymmetrically in a conserved cleft at the interface of the enolase dimer. The recognition site is well conserved in RNase E homologues in a subfamily of the gamma-proteobacteria, including enzymes from pathogens such as Yersinia pestis, Vibrio cholera and Salmonella sp. We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions.
Recognition of enolase in the Escherichia coli RNA degradosome., Chandran V, Luisi BF, J Mol Biol. 2006 Apr 21;358(1):8-15. Epub 2006 Feb 21. PMID:16516921
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2FYM is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Recognition of enolase in the Escherichia coli RNA degradosome., Chandran V, Luisi BF, J Mol Biol. 2006 Apr 21;358(1):8-15. Epub 2006 Feb 21. PMID:16516921
Page seeded by OCA on Tue Jul 29 11:00:38 2008
