2hd5

From Proteopedia

Jump to: navigation, search


2hd5, resolution 1.85Å ()
Ligands:
Gene: USP2, UBP41 (Homo sapiens)
Activity: Ubiquitin thiolesterase, with EC number 3.1.2.15
Related: 1nb8, 1nbf, 2ayn, 2ayo
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

USP2 in complex with ubiquitin

Publication Abstract from PubMed

Deubiquitinating proteases reverse protein ubiquitination and rescue their target proteins from destruction by the proteasome. USP2, a cysteine protease and a member of the ubiquitin specific protease family, is overexpressed in prostate cancer and stabilizes fatty acid synthase, which has been associated with the malignancy of some aggressive prostate cancers. Here, we report the structure of the human USP2 catalytic domain in complex with ubiquitin. Ubiquitin uses two major sites for the interaction with the protease. Both sites are required simultaneously, as shown by USP2 inhibition assays with peptides and ubiquitin mutants. In addition, a layer of ordered water molecules mediates key interactions between ubiquitin and USP2. As several of those molecules are found at identical positions in the previously solved USP7/ubiquitin-aldehyde complex structure, we suggest a general mechanism of water-mediated ubiquitin recognition by USPs.

Structural basis of ubiquitin recognition by the deubiquitinating protease USP2., Renatus M, Parrado SG, D'Arcy A, Eidhoff U, Gerhartz B, Hassiepen U, Pierrat B, Riedl R, Vinzenz D, Worpenberg S, Kroemer M, Structure. 2006 Aug;14(8):1293-302. PMID:16905103

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2hd5 is a 2 chain structure with sequence from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Renatus M, Parrado SG, D'Arcy A, Eidhoff U, Gerhartz B, Hassiepen U, Pierrat B, Riedl R, Vinzenz D, Worpenberg S, Kroemer M. Structural basis of ubiquitin recognition by the deubiquitinating protease USP2. Structure. 2006 Aug;14(8):1293-302. PMID:16905103 doi:10.1016/j.str.2006.06.012

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools