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2hpv
From Proteopedia
| 2hpv, resolution 2.00Å () | |||||||||
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| Non-Standard Residues: | |||||||||
| Gene: | azoR, azoA (Enterococcus faecalis) | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of FMN-Dependent azoreductase from Enterococcus faecalis
The initial critical step of reduction of the azo bond during the metabolism of azo dyes is catalyzed by a group of NAD(P)H dependant enzymes called azoreductases. Although several azoreductases have been identified from microorganisms and partially characterized, very little is known about the structural basis for substrate specificity and the nature of catalysis. Enterococcus faecalis azoreductase A (AzoA) is a highly active azoreductase with a broad spectrum of substrate specificity and is capable of degrading a wide variety of azo dyes. Here, we report the crystal structure of the AzoA from E. faecalis determined at 2.07 A resolution with bound FMN ligand. Phases were obtained by single wavelength anomalous scattering of selenomethionine labeled protein crystals. The asymmetric unit consisted of two dimers with one FMN molecule bound to each monomer. The AzoA monomer takes a typical NAD(P)-binding Rossmann fold with a highly conserved FMN binding pocket. A salt bridge between Arg18 and Asp184 restricts the size of the flavin binding pocket such that only FMN can bind. A putative NADH binding site could be identified and a plausible mechanism for substrate reduction is proposed. Expression studies revealed azoA gene to be expressed constitutively in E. faecalis.
Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalis., Liu ZJ, Chen H, Shaw N, Hopper SL, Chen L, Chen S, Cerniglia CE, Wang BC, Arch Biochem Biophys. 2007 Jul 1;463(1):68-77. Epub 2007 Mar 30. PMID:017428434
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2hpv is a 4 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.
Categories: Enterococcus faecalis | Chen, H. | Chen, L. | Liu, Z J. | Rose, J. | SECSG, Southeast Collaboratory for Structural Genomics. | Wang, B C. | Azoreductase | Enterococcus faecali | Oxidoreductase | Protein structure initiative | Psi | Secsg | Southeast collaboratory for structural genomic | Structural genomic
