2hr9

Publication Abstract from PubMed

Human translationally controlled tumor protein (TCTP) is a growth-related, calcium-binding protein. We determined the solution structure and backbone dynamics of human TCTP, and identified the calcium-binding site of human TCTP using multi-dimensional NMR spectroscopy. The overall structure of human TCTP has a rather rigid well-folded core and a very flexible long loop connected by a short two-strand beta-sheet, which shows a conserved fold in the TCTP family. The C-terminal portions of loop L(alpha3beta8) and strand beta9 and the N-terminal region of strand beta8 may form a calcium-binding site in the human TCTP structure, which is largely conserved in the sequence alignment of TCTPs. The K(d) value for the calcium binding is 0.022-0.025 M indicating a very weak calcium-binding site.

Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR.,Feng Y, Liu D, Yao H, Wang J Arch Biochem Biophys. 2007 Nov 1;467(1):48-57. Epub 2007 Aug 30. PMID:17897616^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.