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|2hyi, resolution 2.30Å ()|
|Gene:||MAGOH (Homo sapiens), RBM8A, RBM8 (Homo sapiens), DDX48, EIF4A3, KIAA0111 (Homo sapiens), CASC3, MLN51 (Homo sapiens)|
|Related:||2hxy, 1p27, 2db3|
Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA
In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA., Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H, Andersen GR, Science. 2006 Sep 29;313(5795):1968-72. Epub 2006 Aug 24. PMID:16931718
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H, Andersen GR. Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA. Science. 2006 Sep 29;313(5795):1968-72. Epub 2006 Aug 24. PMID:16931718