2if1

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HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES

Structural highlights

2if1 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EIF1_HUMAN Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

eIF1 is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. We have determined the solution structure of human eIF1 with an N-terminal His tag using NMR spectroscopy. Residues 29-113 of the native sequence form a tightly packed domain with two alpha-helices on one side of a five-stranded parallel and antiparallel beta-sheet. The fold is new but similar to that of several ribosomal proteins and RNA-binding domains. A likely binding site is indicated by yeast mutations and conserved residues located together on the surface. No interaction with recombinant eIF5 or the initiation site RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show that eIF1 binds specifically to the p110 subunit of eIF3. This interaction explains how eIF1 is recruited to the 40S ribosomal subunit.

Structure and interactions of the translation initiation factor eIF1.,Fletcher CM, Pestova TV, Hellen CU, Wagner G EMBO J. 1999 May 4;18(9):2631-7. PMID:10228174[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
20 reviews cite this structure
The scanning mechanism of eukaryotic translation initiation.
Hinnebusch et al. (2014)
19 more
55 other articles
No citations found

See Also

References

  1. ↑ Fletcher CM, Pestova TV, Hellen CU, Wagner G. Structure and interactions of the translation initiation factor eIF1. EMBO J. 1999 May 4;18(9):2631-7. PMID:10228174 doi:10.1093/emboj/18.9.2631

Contents


PDB ID 2if1

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