2ke8

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2ke8, 20 NMR models ()
Ligands:
Non-Standard Residues:
Related: 2k67, 2k68, 2k69


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



NMR solution structure of metal-modified DNA

Publication Abstract from PubMed

Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from (1)J((1)N,(1)/(1)Ag) couplings upon incorporation of (1)N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs.

Solution structure of a DNA double helix with consecutive metal-mediated base pairs., Johannsen S, Megger N, Bohme D, Sigel RK, Muller J, Nat Chem. 2010 Mar;2(3):229-34. Epub 2010 Jan 17. PMID:21124482

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2ke8 is a 2 chain structure. Full experimental information is available from OCA.

Reference

  • Johannsen S, Megger N, Bohme D, Sigel RK, Muller J. Solution structure of a DNA double helix with consecutive metal-mediated base pairs. Nat Chem. 2010 Mar;2(3):229-34. Epub 2010 Jan 17. PMID:21124482 doi:10.1038/nchem.512

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