Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from (1)J((1)N,(1)/(1)Ag) couplings upon incorporation of (1)N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs.
Solution structure of a DNA double helix with consecutive metal-mediated base pairs.,Johannsen S, Megger N, Bohme D, Sigel RK, Muller J Nat Chem. 2010 Mar;2(3):229-34. Epub 2010 Jan 17. PMID:21124482
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Johannsen S, Megger N, Bohme D, Sigel RK, Muller J. Solution structure of a DNA double helix with consecutive metal-mediated base pairs. Nat Chem. 2010 Mar;2(3):229-34. Epub 2010 Jan 17. PMID:21124482 doi:10.1038/nchem.512