2l0g

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2l0g, 20 NMR models ()
Related: 2l0f


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Solution NMR structure of ubiquitin-binding motif (UBM2) of human polymerase iota

Publication Abstract from PubMed

Cells have evolved mutagenic bypass mechanisms that prevent stalling of the replication machinery at DNA lesions. This process, translesion DNA synthesis (TLS), involves switching from high-fidelity DNA polymerases to specialized DNA polymerases that replicate through a variety of DNA lesions. In eukaryotes, polymerase switching during TLS is regulated by the DNA damage-triggered monoubiquitylation of PCNA. How the switch operates is unknown, but all TLS polymerases of the so-called Y-family possess PCNA and ubiquitin-binding domains that are important for their function. To gain insight into the structural mechanisms underlying the regulation of TLS by ubiquitylation, we have probed the interaction of ubiquitin with a conserved ubiquitin-binding motif (UBM2) of Y-family polymerase Poliota. Using NMR spectroscopy, we have determined the structure of a complex of human Poliota UBM2 and ubiquitin, revealing a novel ubiquitin recognition fold consisting of two alpha-helices separated by a central trans-proline residue conserved in all UBMs. We show that, different from the majority of ubiquitin complexes characterized to date, ubiquitin residue Ile44 only plays a modest role in the association of ubiquitin with Poliota UBM2. Instead, binding of UBM2 is centered on the recognition of Leu8 in ubiquitin, which is essential for the interaction.

Structural Basis of Ubiquitin Recognition by Translesion Synthesis DNA Polymerase iota, Cui G, Benirschke RC, Tuan HF, Juranic N, Macura S, Botuyan MV, Mer G, Biochemistry. 2010 Nov 30;49(47):10198-10207. Epub 2010 Nov 4. PMID:21049971

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2l0g is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.

See Also

Reference

  • Cui G, Benirschke RC, Tuan HF, Juranic N, Macura S, Botuyan MV, Mer G. Structural Basis of Ubiquitin Recognition by Translesion Synthesis DNA Polymerase iota Biochemistry. 2010 Nov 30;49(47):10198-10207. Epub 2010 Nov 4. PMID:21049971 doi:10.1021/bi101303t

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