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2lnk

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2lnk, 20 NMR models ()
Gene: S100A4, CAPL, MTS1 (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Solution structure of Ca-bound S100A4 in complex with non-muscle myosin IIA

Publication Abstract from PubMed

Filament assembly of nonmuscle myosin IIA (NMIIA) is selectively regulated by the small Ca(2+)-binding protein, S100A4, which causes enhanced cell migration and metastasis in certain cancers. Our NMR structure shows that an S100A4 dimer binds to a single myosin heavy chain in an asymmetrical configuration. NMIIA in the complex forms a continuous helix that stretches across the surface of S100A4 and engages the Ca(2+)-dependent binding sites of each subunit in the dimer. Synergy between these sites leads to a very tight association (K(D) approximately 1 nM) that is unique in the S100 family. Single-residue mutations that remove this synergy weaken binding and ameliorate the effects of S100A4 on NMIIA filament assembly and cell spreading in A431 human epithelial carcinoma cells. We propose a model for NMIIA filament disassembly by S100A4 in which initial binding to the unstructured NMIIA tail initiates unzipping of the coiled coil and disruption of filament packing.

Asymmetric Mode of Ca(2+)-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling., Elliott PR, Irvine AF, Jung HS, Tozawa K, Pastok MW, Picone R, Badyal SK, Basran J, Rudland PS, Barraclough R, Lian LY, Bagshaw CR, Kriajevska M, Barsukov IL, Structure. 2012 Apr 4;20(4):654-66. Epub 2012 Apr 3. PMID:22483112

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2lnk is a 3 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.

See Also

Reference

  • Elliott PR, Irvine AF, Jung HS, Tozawa K, Pastok MW, Picone R, Badyal SK, Basran J, Rudland PS, Barraclough R, Lian LY, Bagshaw CR, Kriajevska M, Barsukov IL. Asymmetric Mode of Ca(2+)-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling. Structure. 2012 Apr 4;20(4):654-66. Epub 2012 Apr 3. PMID:22483112 doi:10.1016/j.str.2012.02.002

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