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2qov
From Proteopedia
Contents |
Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 50S subunit of the first 70S ribosome. The entire crystal structure contains two 70S ribosomes.
The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2qov is a 30 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L11
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L28
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L9
Reference
- Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH. A steric block in translation caused by the antibiotic spectinomycin. ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316 doi:10.1021/cb700100n
Categories: Escherichia coli | Borovinskaya, M A. | Cate, J H.D. | Fredrick, K. | Holton, J M. | Shoji, S. | Antibiotic resistance | Methylation | Phosphorylation | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Rna-binding | Rna-protein complex | Rrna-binding | Transcription | Transcription regulation | Transcription termination | Translation regulation | Trna-binding
