2r6c

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Crystal Form BH2

Structural highlights

2r6c is a 9 chain structure with sequence from Bacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:MSE
Gene:DnaB (Bacillus stearothermophilus), DnaG (Bacillus stearothermophilus)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DNAG_GEOSE] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.[HAMAP-Rule:MF_00974]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.

Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase.,Bailey S, Eliason WK, Steitz TA Science. 2007 Oct 19;318(5849):459-63. PMID:17947583[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
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See Also

References

  1. Bailey S, Eliason WK, Steitz TA. Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase. Science. 2007 Oct 19;318(5849):459-63. PMID:17947583 doi:318/5849/459

Contents


PDB ID 2r6c

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