First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
|3ef4, resolution 1.18Å ()|
Crystal structure of native pseudoazurin from Hyphomicrobium denitrificans
The crystal structure of native pseudoazurin (HdPAz) from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans has been determined at a resolution of 1.18 A. After refinement with SHELX employing anisotropic displacement parameters and riding H atoms, R(work) and R(free) were 0.135 and 0.169, respectively. Visualization of the anisotropic displacement parameters as thermal ellipsoids provided insight into the atomic motion within the perturbed type 1 Cu site. The asymmetric unit includes three HdPAz molecules which are tightly packed by head-to-head cupredoxin dimer formation. The shape of the Cu-atom ellipsoid implies significant vibrational motion diagonal to the equatorial xy plane defined by the three ligands (two His and one Cys). The geometric parameters of the type 1 Cu site in the HdPAz structure differ unambiguously from those of other pseudoazurins. It is demonstrated that their structural aspects are consistent with the unique visible absorption spectrum.
Atomic resolution structure of pseudoazurin from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans: structural insights into its spectroscopic properties., Hira D, Nojiri M, Suzuki S, Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):85-92. Epub 2008, Dec 18. PMID:19153470
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Hira D, Nojiri M, Suzuki S. Atomic resolution structure of pseudoazurin from the methylotrophic denitrifying bacterium Hyphomicrobium denitrificans: structural insights into its spectroscopic properties. Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):85-92. Epub 2008, Dec 18. PMID:19153470 doi:10.1107/S0907444908040195