3egz

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3egz, resolution 2.20Å ()
Ligands: ,
Non-Standard Residues:
Gene: SNRPA (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of an in vitro evolved tetracycline aptamer and artificial riboswitch

Publication Abstract from PubMed

The tetracycline aptamer is an in vitro selected RNA that binds to the antibiotic with the highest known affinity of an artificial RNA for a small molecule (Kd approximately 0.8 nM). It is one of few aptamers known to be capable of modulating gene expression in vivo. The 2.2 A resolution cocrystal structure of the aptamer reveals a pseudoknot-like fold formed by tertiary interactions between an 11 nucleotide loop and the minor groove of an irregular helix. Tetracycline binds within this interface as a magnesium ion chelate. The structure, together with previous biochemical and biophysical data, indicates that the aptamer undergoes localized folding concomitant with tetracycline binding. The three-helix junction, h-shaped architecture of this artificial RNA is more complex than those of most aptamers and is reminiscent of the structures of some natural riboswitches.

Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch., Xiao H, Edwards TE, Ferre-D'Amare AR, Chem Biol. 2008 Oct 20;15(10):1125-37. PMID:18940672

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3egz is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Xiao H, Edwards TE, Ferre-D'Amare AR. Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch. Chem Biol. 2008 Oct 20;15(10):1125-37. PMID:18940672 doi:10.1016/j.chembiol.2008.09.004

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