First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
3exb
From Proteopedia
| 3exb, resolution 1.60Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | |||||||||
| Non-Standard Residues: | , | ||||||||
| Gene: | CCP1, CCP, CPO (Saccharomyces cerevisiae) | ||||||||
| Activity: | Cytochrome-c peroxidase, with EC number 1.11.1.5 | ||||||||
| Related: | 1kxn, 1kxm | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal structure of Cytochrome C Peroxidase with a Proposed Electron Pathway Excised in a Complex with a Peptide Wire
A proposed electron transfer pathway in cytochrome c peroxidase was previously excised from the structure by design. The engineered channel mutant was shown to bind peptide surrogates without restoration of cyt c oxidation. Here, we report the 1.6 A crystal structure of (N-benzimidazole-propionic acid)-Gly-Ala-Ala bound within the engineered channel. The peptide retains many features of the native electron transfer pathway: placement of benzimidazole at the position of the Trp-191 radical, hydrogen bonding to Asp235, and positioning of the C-terminus near the point where wild type CcP makes closest contact to cyt c. The inability of this surrogate pathway to restore function supports proposals that electron transfer requires the Trp-191 radical.
Replacement of an electron transfer pathway in cytochrome c peroxidase with a surrogate peptide., Hays Putnam AM, Lee YT, Goodin DB, Biochemistry. 2009 Jan 13;48(1):1-3. PMID:19072042
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3exb is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
See Also
Reference
- Hays Putnam AM, Lee YT, Goodin DB. Replacement of an electron transfer pathway in cytochrome c peroxidase with a surrogate peptide. Biochemistry. 2009 Jan 13;48(1):1-3. PMID:19072042 doi:10.1021/bi8020263
