3hhn

From Proteopedia

Jump to: navigation, search


3hhn, resolution 2.99Å ()
Ligands:
Non-Standard Residues:
Gene: SNRPA (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of class I ligase ribozyme self-ligation product, in complex with U1A RBD

Publication Abstract from PubMed

Primordial organisms of the putative RNA world would have required polymerase ribozymes able to replicate RNA. Known ribozymes with polymerase activity best approximating that needed for RNA replication contain at their catalytic core the class I RNA ligase, an artificial ribozyme with a catalytic rate among the fastest of known ribozymes. Here we present the 3.0 angstrom crystal structure of this ligase. The architecture resembles a tripod, its three legs converging near the ligation junction. Interacting with this tripod scaffold through a series of 10 minor-groove interactions (including two A-minor triads) is the unpaired segment that contributes to and organizes the active site. A cytosine nucleobase and two backbone phosphates abut the ligation junction; their location suggests a model for catalysis resembling that of proteinaceous polymerases.

Crystal structure of the catalytic core of an RNA-polymerase ribozyme., Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, Bartel DP, Science. 2009 Nov 27;326(5957):1271-5. PMID:19965478

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3hhn is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, Bartel DP. Crystal structure of the catalytic core of an RNA-polymerase ribozyme. Science. 2009 Nov 27;326(5957):1271-5. PMID:19965478 doi:326/5957/1271

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools