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3i1n
From Proteopedia
Contents |
Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
Protein biosynthesis on the ribosome requires repeated cycles of ratcheting, which couples rotation of the two ribosomal subunits with respect to each other, and swiveling of the head domain of the small subunit. However, the molecular basis for how the two ribosomal subunits rearrange contacts with each other during ratcheting while remaining stably associated is not known. Here, we describe x-ray crystal structures of the intact Escherichia coli ribosome, either in the apo-form (3.5 angstrom resolution) or with one (4.0 angstrom resolution) or two (4.0 angstrom resolution) anticodon stem-loop tRNA mimics bound, that reveal intermediate states of intersubunit rotation. In the structures, the interface between the small and large ribosomal subunits rearranges in discrete steps along the ratcheting pathway. Positioning of the head domain of the small subunit is controlled by interactions with the large subunit and with the tRNA bound in the peptidyl-tRNA site. The intermediates observed here provide insight into how tRNAs move into the hybrid state of binding that precedes the final steps of mRNA and tRNA translocation.
Structures of the ribosome in intermediate states of ratcheting., Zhang W, Dunkle JA, Cate JH, Science. 2009 Aug 21;325(5943):1014-7. PMID:19696352
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3i1n is a 30 chain structure with sequence from Escherichia coli and Escherichia coli k-12. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L11
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L28
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L9
- Ribosome
Reference
- Zhang W, Dunkle JA, Cate JH. Structures of the ribosome in intermediate states of ratcheting. Science. 2009 Aug 21;325(5943):1014-7. PMID:19696352 doi:325/5943/1014
Categories: Escherichia coli | Escherichia coli k-12 | Cate, J H.D. | Dunkle, J A. | Zhang, W. | Antibiotic resistance | Methylation | Phosphoprotein | Protein-rna complex | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Ribosome structure | Rna-binding | Rrna-binding | Transcription | Transcription regulation | Transcription termination | Translation regulation | Trna-binding
