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3i1s
From Proteopedia
Contents |
Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
Protein biosynthesis on the ribosome requires repeated cycles of ratcheting, which couples rotation of the two ribosomal subunits with respect to each other, and swiveling of the head domain of the small subunit. However, the molecular basis for how the two ribosomal subunits rearrange contacts with each other during ratcheting while remaining stably associated is not known. Here, we describe x-ray crystal structures of the intact Escherichia coli ribosome, either in the apo-form (3.5 angstrom resolution) or with one (4.0 angstrom resolution) or two (4.0 angstrom resolution) anticodon stem-loop tRNA mimics bound, that reveal intermediate states of intersubunit rotation. In the structures, the interface between the small and large ribosomal subunits rearranges in discrete steps along the ratcheting pathway. Positioning of the head domain of the small subunit is controlled by interactions with the large subunit and with the tRNA bound in the peptidyl-tRNA site. The intermediates observed here provide insight into how tRNAs move into the hybrid state of binding that precedes the final steps of mRNA and tRNA translocation.
Structures of the ribosome in intermediate states of ratcheting., Zhang W, Dunkle JA, Cate JH, Science. 2009 Aug 21;325(5943):1014-7. PMID:19696352
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3i1s is a 23 chain structure with sequence from Escherichia coli and Escherichia coli k-12. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein S10
- Ribosomal protein S11
- Ribosomal protein S12
- Ribosomal protein S13
- Ribosomal protein S14
- Ribosomal protein S15
- Ribosomal protein S16
- Ribosomal protein S17
- Ribosomal protein S18
- Ribosomal protein S19
- Ribosomal protein S2
- Ribosomal protein S20
- Ribosomal protein S21
- Ribosomal protein S3
- Ribosomal protein S4
- Ribosomal protein S5
- Ribosomal protein S6
- Ribosomal protein S7
- Ribosomal protein S8
- Ribosomal protein S9
- Ribosome
Reference
- Zhang W, Dunkle JA, Cate JH. Structures of the ribosome in intermediate states of ratcheting. Science. 2009 Aug 21;325(5943):1014-7. PMID:19696352 doi:325/5943/1014
Categories: Escherichia coli | Escherichia coli k-12 | Cate, J H.D. | Dunkle, J A. | Zhang, W. | Antibiotic resistance | Endonuclease | Hydrolase | Methylation | Nuclease | Protein-rna complex | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Ribosome structure | Rna-binding | Rrna-binding | Transcription | Transcription regulation | Transcription termination | Translation regulation | Trna-binding
