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3i8i
From Proteopedia
Contents
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Elongation complex of the 70S ribosome with three tRNAs and mRNA. This entry 3I8I contains 50S ribosomal subnit. The 30S ribosomal subunit can be found in PDB entry 3I8H. Molecule A in the same asymmetric unit is deposited as 3I8F (50S) and 3I8G (30S).
One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.
Structural aspects of messenger RNA reading frame maintenance by the ribosome., Jenner LB, Demeshkina N, Yusupova G, Yusupov M, Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3i8i is a 33 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
- Gramicidin
- Ribosomal protein L10
- Ribosomal protein L11
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L31
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L7
- Ribosomal protein L9
Reference
- Jenner LB, Demeshkina N, Yusupova G, Yusupov M. Structural aspects of messenger RNA reading frame maintenance by the ribosome. Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952 doi:10.1038/nsmb.1790
- Jenner L, Demeshkina N, Yusupova G, Yusupov M. Structural rearrangements of the ribosome at the tRNA proofreading step. Nat Struct Mol Biol. 2010 Sep;17(9):1072-8. Epub 2010 Aug 8. PMID:20694005 doi:10.1038/nsmb.1880
