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3ipm
From Proteopedia
| 3ipm, resolution 4.00Å () | |||||||||
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| Gene: | psmA, Ta1288 (Thermoplasma acidophilum), psmB, Ta0612 (Thermoplasma acidophilum), Tb10.70.3660 (Methanocaldococcus jannaschii) | ||||||||
| Activity: | Proteasome endopeptidase complex, with EC number 3.4.25.1 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN
Protein degradation in the 20S proteasome is regulated in eukaryotes by the 19S ATPase complex and in archaea by the homologous PAN ATPase ring complex. Subunits of these hexameric ATPases contain on their C-termini a conserved hydrophobic-tyrosine-X (HbYX) motif that docks into pockets in the 20S to stimulate the opening of a gated substrate entry channel. Here, we report the crystal structure of the archaeal 20S proteasome in complex with the C-terminus of the archaeal proteasome regulatory ATPase, PAN. This structure defines the detailed interactions between the critical C-terminal HbYX motif and the 20S alpha-subunits and indicates that the intersubunit pocket in the 20S undergoes an induced-fit conformational change on binding of the HbYX motif. This structure together with related mutagenesis data suggest how in eukaryotes certain proteasomal ATPases bind to specific pockets in an asymmetrical manner to regulate gate opening.
Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions., Yu Y, Smith DM, Kim HM, Rodriguez V, Goldberg AL, Cheng Y, EMBO J. 2010 Feb 3;29(3):692-702. Epub 2009 Dec 17. PMID:20019667
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3ipm is a 21 chain structure with sequence from Methanocaldococcus jannaschii and Thermoplasma acidophilum. Full crystallographic information is available from OCA.
See Also
Reference
- Yu Y, Smith DM, Kim HM, Rodriguez V, Goldberg AL, Cheng Y. Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions. EMBO J. 2010 Feb 3;29(3):692-702. Epub 2009 Dec 17. PMID:20019667 doi:10.1038/emboj.2009.382
Categories: Methanocaldococcus jannaschii | Proteasome endopeptidase complex | Thermoplasma acidophilum | Cheng, Y. | Yu, Y. | Aaa atpase | Electron cryomicroscopy | Hydrolase | Hydrolase-hydrolase activator complex | Protease | Proteasomal atpase | Proteasome | Protein degradation | Threonine protease
