3ldz

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3ldz, resolution 2.60Å ()
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of human STAM1 VHS domain in complex with ubiquitin

Publication Abstract from PubMed

VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2.6 A resolution, revealing that determinants for ubiquitin recognition are conserved in nearly all VHS domains. VHS domains from all classes of VHS-domain containing proteins in yeast and humans, including both subunits of ESCRT-0, bound ubiquitin in vitro. ESCRTs have been implicated in the sorting of Lys63-linked polyubiquitinated cargo. Intact human ESCRT-0 binds Lys63-linked tetraubiquitin 50-fold more tightly than monoubiquitin, though only 2-fold more tightly than Lys48-linked tetraubiquitin. The gain in affinity is attributed to the cooperation of flexibly connected VHS and UIM motifs of ESCRT-0 in avid binding to the polyubiquitin chain. Mutational analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that cooperation between them is required for the sorting of the Lys63-linked polyubiquitinated cargo Cps1 to the vacuole.

VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo., Ren X, Hurley JH, EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. PMID:20150893

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3ldz is a 7 chain structure with sequence from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Ren X, Hurley JH. VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. PMID:20150893 doi:10.1038/emboj.2010.6

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