3lk3

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3lk3, resolution 2.68Å ()
Gene: CAPZA1 (Gallus gallus), CAPZB (Gallus gallus), CARMIL, LRRC16, LRRC16A (Homo sapiens)
Related: 3lk2, 3lk4


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of CapZ bound to the CPI and CSI uncapping motifs from CARMIL

Publication Abstract from PubMed

Capping protein (CP) regulates actin dynamics by binding the barbed ends of actin filaments. Removal of CP may be one means to harness actin polymerization for processes such as cell movement and endocytosis. Here we structurally and biochemically investigated a CP interaction (CPI) motif present in the otherwise unrelated proteins CARMIL and CD2AP. The CPI motif wraps around the stalk of the mushroom-shaped CP at a site distant from the actin-binding interface, which lies on the top of the mushroom cap. We propose that the CPI motif may act as an allosteric modulator, restricting CP to a low-affinity, filament-binding conformation. Structure-based sequence alignments extend the CPI motif-containing family to include CIN85, CKIP-1, CapZIP and a relatively uncharacterized protein, WASHCAP (FAM21). Peptides comprising these CPI motifs are able to inhibit CP and to uncap CP-bound actin filaments.

Structural characterization of a capping protein interaction motif defines a family of actin filament regulators., Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC, Nat Struct Mol Biol. 2010 Apr;17(4):497-503. Epub 2010 Mar 28. PMID:020357771

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3lk3 is a 3 chain structure with sequence from Gallus gallus and Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC. Structural characterization of a capping protein interaction motif defines a family of actin filament regulators. Nat Struct Mol Biol. 2010 Apr;17(4):497-503. Epub 2010 Mar 28. PMID:20357771 doi:10.1038/nsmb.1792

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