3rto

From Proteopedia

Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure

Structural highlights

3rto is a 4 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

INS_PIG Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Publication Abstract from PubMed

We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 A resolution crystal structures.

Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures.,Soares AS, Engel MA, Stearns R, Datwani S, Olechno J, Ellson R, Skinner JM, Allaire M, Orville AM Biochemistry. 2011 May 9. PMID:21542590^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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References

↑ Soares AS, Engel MA, Stearns R, Datwani S, Olechno J, Ellson R, Skinner JM, Allaire M, Orville AM. Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures. Biochemistry. 2011 May 9. PMID:21542590 doi:10.1021/bi200549x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

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PDB ID 3rto

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3rto

From Proteopedia

Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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