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4fgb, resolution 2.60Å ()
Gene: CAMK1 (Homo sapiens)
Activity: Calcium/calmodulin-dependent protein kinase, with EC number
Related: 4fg7, 4fg8, 4fg9

Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml

Crystal structure of human calcium/calmodulin-dependent protein kinase I apo form

Publication Abstract from PubMed

Human calcium/calmodulin-dependent protein kinase I (CaMKI) plays pivotal roles in the nervous system. The activity of human CaMKI is regulated by a regulatory region including an autoinhibitory segment and a CaM-binding segment. We report here four structures of three CaMKIalpha truncates in apo form and in complexes with ATP. In an apo, autoinhibited structure, the activation segment adopts a unique helical conformation which together with the autoinhibitory segment constrains helices alphaC and alphaD in inactive conformations, sequesters Thr177 from being phosphorylated, and occludes the substrate-binding site. In an ATP-bound, inactive structure, the activation segment is largely disordered and the CaM-binding segment protrudes out ready for CaM binding. In an ATP-bound, active structure, the regulatory region is dissociated from the catalytic core and the catalytic site assumes an active conformation. Detailed structural analyses reveal the interplay of the regulatory region, the activation segment, and the nucleotide-binding site in the regulation of CaMKI.

Crystal structures of human CaMKIalpha reveal insights into the regulation mechanism of CaMKI., Zha M, Zhong C, Ou Y, Han L, Wang J, Ding J, PLoS One. 2012;7(9):e44828. doi: 10.1371/journal.pone.0044828. Epub 2012 Sep 20. PMID:23028635

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

4fgb is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

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