4m3w

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4m3w, resolution 2.10Å ()
Ligands: ,
Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7
Related: 4m3r, 4m3t, 4m3u


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

RB69 DNA polymerase ternary complex with dT/dG at position n-4 of primer/template duplex

Publication Abstract from PubMed

Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson-Crick geometry in the presence of Mn2+ indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base-pair could be affect by its microenvironment, we determined ten structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n-1 to n-5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson-Crick geometry, have been observed, strongly suggesting that the local environment of base-pairs plays an important role in their tautomeric or ionization states.

Mispairs with Watson-Crick Base-pair Geometry Observed in Ternary Complexes of an RB69 DNA Polymerase Variant., Xia S, Konigsberg WH, Protein Sci. 2014 Jan 24. doi: 10.1002/pro.2434. PMID:24458997

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Function

[DPOL_BPR69] This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction.

About this Structure

4m3w is a 3 chain structure. Full crystallographic information is available from OCA.

See Also

Reference

  • Xia S, Konigsberg WH. Mispairs with Watson-Crick Base-pair Geometry Observed in Ternary Complexes of an RB69 DNA Polymerase Variant. Protein Sci. 2014 Jan 24. doi: 10.1002/pro.2434. PMID:24458997 doi:http://dx.doi.org/10.1002/pro.2434

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