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Publication Abstract from PubMed

We have previously reported a phage display method for the identification of protein domains on a genome-wide scale (shotgun proteolysis). Here we present the solution structure of a fragment of the Escherichia coli membrane protein yrfF, as identified by shotgun proteolysis, and determined by NMR spectroscopy. Despite the absence of computational predictions, the fragment formed a well-defined beta-barrel structure, distantly falling within the OB-fold classification. Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies.

Solution structure of a soluble fragment derived from a membrane protein by shotgun proteolysis.,Allen MD, Christie M, Jones P, Porebski BT, Roome B, Freund SM, Buckle AM, Bycroft M, Christ D Protein Eng Des Sel. 2015 Apr 15. pii: gzv021. PMID:25877662^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.