5ams
From Proteopedia
Crystal structure of Sqt1
Structural highlights
FunctionSQT1_YEAST May be involved in the late step of 60S ribosomal subunit assembly or modification in the cytoplasm. Publication Abstract from PubMedRibosome biogenesis in eukaryotes is a complex and highly orchestrated process involving more than 200 accessory factors in addition to ribosomal RNAs and ribosomal proteins. Among the many factors involved, Sqt1p has been reported to specifically bind to uL16 and to act as a chaperone. The crystal structure of full-length Sqt1p from the yeast Saccharomyces cerevisiae has been solved at 3.35 A resolution. A SAD experiment at the Se K edge and an S-SAD experiment on the same selenomethionine-substituted protein crystal allowed unambiguous positioning of the selenomethionine and Cys residues. On the basis of the atomic structure of Sqt1p, the potential residues involved in uL16 interaction were identified and tested. Sqt1p is an eight-bladed WD40 protein.,Frenois F, Legrand P, Fribourg S Acta Crystallogr F Struct Biol Commun. 2016 Jan 1;72(Pt 1):59-64. doi:, 10.1107/S2053230X15024097. Epub 2016 Jan 1. PMID:26750486[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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