5vdi

From Proteopedia

Crystal Structure of Human Glycine Receptor alpha-3 Mutant N38Q Bound to AM-3607, Glycine, and Ivermectin

PDB ID 5vdi

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Structural highlights

5vdi is a 5 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.1Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLRA3_HUMAN The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

Publication Abstract from PubMed

Ivermectin acts as a positive allosteric modulator of several Cys-loop receptors including the glutamate-gated chloride channels (GluCls), gamma-aminobutyric acid receptors (GABAARs), glycine receptors (GlyRs), and neuronal alpha7-nicotinic receptors (alpha7 nAChRs). The crystal structure of Caenorhabditis elegans GluCl complexed with ivermectin revealed the details of its ivermectin binding site. Although the electron microscopy structure of zebrafish GlyRalpha1 complexed with ivermectin demonstrated a similar binding orientation, detailed structural information on the ivermectin binding and pore opening for Cys-loop receptors in vertebrates has been elusive. Here we present the crystal structures of human GlyRalpha3 in complex with ivermectin at 2.85 and 3.08 A resolution. Our structures allow us to explore in detail the molecular recognition of ivermectin by GlyRs, GABAARs, and alpha7 nAChRs. Comparisons with previous structures reveal how the ivermectin binding expands the ion channel pore. Our results hold promise in structure-based design of GlyR modulators for the treatment of neuropathic pain.

Crystal Structures of Human GlyRalpha3 Bound to Ivermectin.,Huang X, Chen H, Shaffer PL Structure. 2017 May 4. pii: S0969-2126(17)30108-9. doi:, 10.1016/j.str.2017.04.007. PMID:28479061^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huang X, Chen H, Shaffer PL. Crystal Structures of Human GlyRalpha3 Bound to Ivermectin. Structure. 2017 May 4. pii: S0969-2126(17)30108-9. doi:, 10.1016/j.str.2017.04.007. PMID:28479061 doi:http://dx.doi.org/10.1016/j.str.2017.04.007