Structural highlights
5wri is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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Ligands: | , , , |
Related: | 3ap1, 5wrj |
Activity: | Protein-tyrosine sulfotransferase, with EC number 2.8.2.20 |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[TPST1_HUMAN] Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Publication Abstract from PubMed
Tyrosylprotein sulfotransferases (TPSTs) are enzymes that catalyze post-translational tyrosine sulfation of proteins. In humans, there are only two TPST isoforms, designated TPST1 and TPST2. In a previous study, we reported the crystal structure of TPST2, which revealed the catalytic mechanism of the tyrosine sulfation reaction. However, detailed molecular mechanisms underlying how TPSTs catalyse a variety of substrate proteins with different efficiencies and how TPSTs catalyze the sulfation of multiple tyrosine residues in a substrate protein remain unresolved. Here, we report two crystal structures of the human TPST1 complexed with two substrate peptides that are catalysed by human TPST1 with significantly different efficiencies. The distinct binding modes found in the two complexes provide insight into the sulfation mechanism for these substrates. The present study provides valuable information describing the molecular mechanism of post-translational protein modifications catalysed by TPSTs.
Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1.,Tanaka S, Nishiyori T, Kojo H, Otsubo R, Tsuruta M, Kurogi K, Liu MC, Suiko M, Sakakibara Y, Kakuta Y Sci Rep. 2017 Aug 18;7(1):8776. doi: 10.1038/s41598-017-07141-8. PMID:28821720[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tanaka S, Nishiyori T, Kojo H, Otsubo R, Tsuruta M, Kurogi K, Liu MC, Suiko M, Sakakibara Y, Kakuta Y. Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1. Sci Rep. 2017 Aug 18;7(1):8776. doi: 10.1038/s41598-017-07141-8. PMID:28821720 doi:http://dx.doi.org/10.1038/s41598-017-07141-8