Structural highlights
Publication Abstract from PubMed
The epithelial sodium channel (ENaC), a member of the ENaC/DEG superfamily, regulates Na(+) and water homeostasis. ENaCs assemble as heterotrimeric channels that harbor protease-sensitive domains critical for gating the channel. Here, we present the structure of human ENaC in the uncleaved state determined by single-particle cryo-electron microscopy. The ion channel is composed of a large extracellular domain and a narrow transmembrane domain. The structure reveals that ENaC assembles with a 1:1:1 stoichiometry of alpha:beta:gamma subunits arranged in a counter-clockwise manner. The shape of each subunit is reminiscent of a hand with key gating domains of a 'finger' and a 'thumb.' Wedged between these domains is the elusive protease-sensitive inhibitory domain poised to regulate conformational changes of the 'finger' and 'thumb'; thus, the structure provides the first view of the architecture of inhibition of ENaC.
Structure of the human epithelial sodium channel by cryo-electron microscopy.,Noreng S, Bharadwaj A, Posert R, Yoshioka C, Baconguis I Elife. 2018 Sep 25;7. pii: 39340. doi: 10.7554/eLife.39340. PMID:30251954[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Noreng S, Bharadwaj A, Posert R, Yoshioka C, Baconguis I. Structure of the human epithelial sodium channel by cryo-electron microscopy. Elife. 2018 Sep 25;7. pii: 39340. doi: 10.7554/eLife.39340. PMID:30251954 doi:http://dx.doi.org/10.7554/eLife.39340