6g32
From Proteopedia
Crystal structure of human geranylgeranyl diphosphate synthase mutant D188Y
Structural highlights
Function[GGPPS_HUMAN] Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins. Publication Abstract from PubMedBisphosphonates are widely used for treating osteoporosis, a common disorder in which bone strength is reduced, increasing the risk for fractures. Rarely, bisphosphonates can paradoxically lead to atypical fractures occurring spontaneously or with trivial trauma. Recently, a novel missense mutation (D188Y) in the GGPS1 gene, encoding for geranylgeranyl diphosphate synthase (GGPPS), was associated with bisphosphonates- induced atypical fractures. However, the molecular basis for GGPPS involvement in this devastating condition remains elusive. Here, we show that while maintaining an overall unperturbed global enzyme structure, the D188Y mutation leads to a ~4-fold catalytic activity decrease. Furthermore, GGPPS-D188Y is unable to support cross-species complementation, highlighting the functional significance of the reduced catalytic activity observed in vitro. We next determined the crystal structure of apo GGPPS-D188Y, revealing that while Y188 does not alter the protein fold, its bulky side-chain sterically interferes with substrate binding. In agreement, we show that GGPPS-D188Y exhibits ~3-fold reduction in the binding affinity of zoledronate, a commonly used bisphosphonate. However, inhibition of the mutated enzyme by zoledronate, in pharmacologically-relevant concentrations, is maintained. Finally, we determined the crystal structure of zoledronate-bound GGPPS-D188Y, revealing large ligand- induced binding pocket rearrangements, revising the previous model for GGPPS- bisphosphonates interaction. In conclusion, we propose that among heterozygotes residual GGPPS activity is sufficient to support physiological cellular function, concealing any pathologic phenotype. However, under bisphosphonates treatment, GGPPS activity is reduced below a crucial threshold for osteoclasts function, leading to impaired bone remodeling and increased susceptibility to atypical fractures. Reduced Activity of GGPPS Mutant is Involved in Bisphosphonates-Induced Atypical Fractures.,Lisnyansky M, Kapelushnik N, Ben-Bassat A, Marom M, Loewenstein A, Khananshvili D, Giladi M, Haitin Y Mol Pharmacol. 2018 Oct 1. pii: mol.118.113670. doi: 10.1124/mol.118.113670. PMID:30275041[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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