6om2

From Proteopedia

Crystal structure of atypical integrin alphaV beta8 with proTGF-beta1 ligand peptide

PDB ID 6om2

Drag the structure with the mouse to rotate

Structural highlights

6om2 is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.77Å
Ligands:	, , , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ITAV_HUMAN The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Publication Abstract from PubMed

Integrin alphaVbeta8, which like alphaVbeta6 functions to activate TGF-betas, is atypical. Its beta8 subunit binds to a distinctive cytoskeleton adaptor and does not exhibit large changes in conformation upon binding to ligand. Here, crystal structures, hydrogen-deuterium exchange dynamics, and affinity measurements on mutants are used to compare alphaVbeta8 and alphaVbeta6. Lack of a binding site for one of three betaI domain divalent cations and a unique beta6-alpha7 loop conformation in beta8 facilitate movements of the alpha1 and alpha1' helices at the ligand binding pocket toward the high affinity state, without coupling to beta6-alpha7 loop reshaping and alpha7-helix pistoning that drive large changes in betaI domain-hybrid domain orientation seen in other integrins. Reciprocal swaps between beta6 and beta8 betaI domains increase affinity of alphaVbeta6 and decrease affinity of alphaVbeta8 and define features that regulate affinity of the betaI domain and its coupling to the hybrid domain.

General structural features that regulate integrin affinity revealed by atypical alphaVbeta8.,Wang J, Su Y, Iacob RE, Engen JR, Springer TA Nat Commun. 2019 Dec 2;10(1):5481. doi: 10.1038/s41467-019-13248-5. PMID:31792290^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang J, Su Y, Iacob RE, Engen JR, Springer TA. General structural features that regulate integrin affinity revealed by atypical alphaVbeta8. Nat Commun. 2019 Dec 2;10(1):5481. doi: 10.1038/s41467-019-13248-5. PMID:31792290 doi:http://dx.doi.org/10.1038/s41467-019-13248-5