7b75
From Proteopedia
Cryo-EM Structure of Human Thyroglobulin
Structural highlights
Disease[THYG_HUMAN] Familial thyroid dyshormonogenesis. The disease is caused by mutations affecting the gene represented in this entry. Disease susceptibility is associated with variations affecting the gene represented in this entry. Function[THYG_HUMAN] Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Publication Abstract from PubMedThe thyroglobulin (TG) protein is essential to thyroid hormone synthesis, plays a vital role in the regulation of metabolism, development and growth and serves as intraglandular iodine storage. Its architecture is conserved among vertebrates. Synthesis of triiodothyronine (T3) and thyroxine (T4) hormones depends on the conformation, iodination and post-translational modification of TG. Although structural information is available on recombinant and deglycosylated endogenous human thyroglobulin (hTG) from patients with goiters, the structure of native, fully glycosylated hTG remained unknown. Here, we present the cryo-electron microscopy structure of native and fully glycosylated hTG from healthy thyroid glands to 3.2 A resolution. The structure provides detailed information on hormonogenic and glycosylation sites. We employ liquid chromatography-mass spectrometry (LC-MS) to validate these findings as well as other post-translational modifications and proteolytic cleavage sites. Our results offer insights into thyroid hormonogenesis of native hTG and provide a fundamental understanding of clinically relevant mutations. Cryo-EM structure of native human thyroglobulin.,Adaixo R, Steiner EM, Righetto RD, Schmidt A, Stahlberg H, Taylor NMI Nat Commun. 2022 Jan 10;13(1):61. doi: 10.1038/s41467-021-27693-8. PMID:35013249[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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