Carbohydrate binding domain from Streptococcus pneumoniae NanA sialidase complexed with 3'-sialyllactose

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Neuraminidase carbohydrate-binding domain complex with 3-sialyllactose and TRIS (PDB code 4c1w)

Contents

Function

This sialidase was designed and tested to protect mice from H1N1 by masking cell surface sialic acid receptors recognized by influenza virion, preventing viral binding and entry into the host cells.

Relevance

Influenza binds to sialic acid receptors present on the respiratory epithelium, eventually gaining access through endocytosis.[1] By potentially concealing sialic acid receptors, it has been proposed that a sialic acid binding protein would aid in prevention of influenza infection. [2]

Structural highlights

The carbohydrate binding molecule (CBM) has a ligand chemical component identified with Tris(Hydroxymethyl)Aminomethane, an organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals. This interaction can be see through the . Lactose sialic acid is another identifier and ligand of the CBM . Close interaction between lactose sialic acid and the carbohydrate binding molecule can be seen through and .


Loc 112-119 interacting with TRS
Loc 112-119 interacting with TRS
Loc 47-72 interaction with SLT
Loc 47-72 interaction with SLT

References

  1. SkehelJJ, Wiley DC (2000) Receptor binding and Membrane fusion in virus entry: The influenza hemagglutinin. Annu Rev Biochem 69:531-569
  2. Connaris H, Govorkova EA, Ligertwood Y, Dutia BM, Yang L, Tauber S, et al. Prevention of influenza by targeting host receptors using engineered proteins. Proc Natl Acad Sci U S A. 2014;111(17):6401–6406. doi: 10.1073/pnas.1404205111

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Michal Harel, Alexander Clare

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